Deleted
Sample Discontinued
Experience: None
Not Used
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DNA

Part:BBa_K2100046:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pBM3R1:mKate


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 242
    Illegal NheI site found at 115
    Illegal NotI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 235
    Illegal BsaI.rc site found at 906
    Illegal SapI.rc site found at 288


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a promoter from a mammalian genome and a gene from an anemone genome.

References